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We conducted a longitudinal surveillance study of bat SL-CoVs using quantitative real-time PCR (qRT-PCR) targeting the nucleocapsid (N) and RNA-dependent RNA polymerase (RdRp) genes in one bat population in Yunnan, China. A total of 431 bat fecal samples were collected during 2011–2014.Fifty-seven of 431 bat fecal samples were positive for SL-CoVs by RT-PCR screening. The detection rate varied significantly among sampling dates (ANOVA,F=28.42, P= 0.03), from 3.1% to 48.7%. The N and RdRp assays revealed dynamic changes in SL-CoV concentrations in the longitudinal surveillance. Fecal pellets with high virus concentrations were typically collected in either September 2012 or July 2013. Additionally, the average virus concentrations in July, September, and October were over 5-fold higher than those observed in April and May. These results indicated that SL-CoV amplification was more efficient from the late summer to autumn. (Letter to the editor)
Coronaviruses, SARS (Disease), Bats -- Viruses, Polymerase chain reaction, Asia, China, Yunnan
Asia; China; Yunnan
Volume 31, Issue 1
Wang, Mei-Niang; Zhang, Wei; and Gao, Yu-Tao, "Longitudinal surveillance of SARS-like coronaviruses in bats by quantitative real-time PCR" (2016). KIP Articles. 3231.