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Seeds of the endangered species Zizania texana are recalcitrant, making it difficult to preserve the remaining genetic diversity of this species in genebanks. Excised embryos can be cryopreserved using solution-based cryoprotection protocols. Survival following cryoexposure increased from less than 5% to about 75% by preculturing embryos in high concentrations of sugars, bathing them in cryoprotectant solutions, and partially drying them to water contents of about 0.6 g H2O/g dry mass.
Cryopreservation, Dessication Tolerance, Embryo, Recalcitrant, Vitrification, Wildrice, Zizania
Cryo letters, Vol. 23, no. 5 (2002-05).
Walters, Christina; Touchell, Darren; Power, Paula; Wesley-Smith, James; and Antolin, Michael, "A cryopreservation protocol for embryos of the endangered species Zizania texana." (2002). KIP Articles. 1168.