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Publisher

PubMed

Publication Date

May 2002

Abstract

Seeds of the endangered species Zizania texana are recalcitrant, making it difficult to preserve the remaining genetic diversity of this species in genebanks. Excised embryos can be cryopreserved using solution-based cryoprotection protocols. Survival following cryoexposure increased from less than 5% to about 75% by preculturing embryos in high concentrations of sugars, bathing them in cryoprotectant solutions, and partially drying them to water contents of about 0.6 g H2O/g dry mass.

Notes

Cryo letters, Vol. 23, no. 5 (2002-05).

Keywords

Cryopreservation, Dessication Tolerance, Embryo, Recalcitrant, Vitrification, Wildrice, Zizania

Description

RDA

Subject: topical

Cryopreservation; Dessication Tolerance; Embryo; Recalcitrant; Vitrification; Wildrice; Zizania

Type

Article

Genre

serial

Identifier

K26-05219

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