Title

A cryopreservation protocol for embryos of the endangered species Zizania texana.

Creator

Christina Walters
Darren Touchell
Paula Power
James Wesley-Smith
Michael Antolin

Files

Publication Date

May 2002

Abstract

Seeds of the endangered species Zizania texana are recalcitrant, making it difficult to preserve the remaining genetic diversity of this species in genebanks. Excised embryos can be cryopreserved using solution-based cryoprotection protocols. Survival following cryoexposure increased from less than 5% to about 75% by preculturing embryos in high concentrations of sugars, bathing them in cryoprotectant solutions, and partially drying them to water contents of about 0.6 g H2O/g dry mass.

Keywords

Cryopreservation, Dessication Tolerance, Embryo, Recalcitrant, Vitrification, Wildrice, Zizania

Type

Article

Notes

Cryo letters, Vol. 23, no. 5 (2002-05).

Publisher

PubMed

Identifier

K26-05219

Recommended Citation

Walters, Christina; Touchell, Darren; Power, Paula; Wesley-Smith, James; and Antolin, Michael, "A cryopreservation protocol for embryos of the endangered species Zizania texana." (2002). KIP Articles. 1168.
https://digitalcommons.usf.edu/kip_articles/1168