Graduation Year


Document Type




Degree Name

Doctor of Philosophy (Ph.D.)

Degree Granting Department


Major Professor

Anna Pyayt, Ph.D.

Committee Member

Rays Jiang, Ph.D.

Committee Member

Sylvia Thomas, Ph.D.

Committee Member

Christopher Passaglia, Ph.D.

Committee Member

Yusuf Emirov, Ph.D.


Biomedical Testing, Image Processing, Incubators, Instrumentation, Thermochromic Polymers


Enzyme-linked immunosorbent assay (ELISA) is one of the most important technologies for biochemical analysis critical for diagnosis and monitoring of many diseases. Traditional systems for ELISA incubation and reading are expensive and bulky, thus cannot be used at pointof- care or in the field. Here, we design and demonstrate a new miniature mobile phone based system for ELISA. This mHealth system can be used to complete all steps of the assay, including incubation and reading. It can be fabricated at low cost, portable, and can transfer test results via mobile phone. We have designed the sample dispensing system, incubation chamber, imaging enclosure, and data processing algorithm. We demonstrate how mobile ELISA can be calibrated for accurate measurements of progesterone and show successful measurements with the calibrated system. We show that the results acquired with our prototype match the gold standard plate reader within 10%. The results satisfy the point-of-care requirements and can be implemented in clinical trials.