Graduation Year
2023
Document Type
Dissertation
Degree
Ph.D.
Degree Name
Doctor of Philosophy (Ph.D.)
Degree Granting Department
Chemistry
Major Professor
Wayne Guida, Ph.D.
Committee Member
James Leahy, Ph.D.
Committee Member
Kenyon Daniel, Ph.D.
Committee Member
Yu Chen, Ph.D.
Keywords
Stimulator of Interferon Genes, Clonixeril, High Pressure Liquid Chromatography, Microscale Thermophoresis, Dynamic Light Scattering, Drug Discovery
Abstract
This work elaborates on analytical and biophysical techniques including high pressure liquid chromatography (HPLC), mass spectrometry quadrupole time-of-flight (MS-QToF), dynamic light scattering (DLS), microscale thermophoresis (MST), surface plasmon resonance (SPR), isothermal calorimetry (ITC), size exclusion chromatography, and native gel electrophoresis. A basic overview is described for each technique and is designed for easy understanding by both novice and experienced analysts. Best practices for each technique are discussed and suggestions are made for protocol improvements or troubleshooting. Protocol development is discussed, and a method is introduced for MST that can be adapted to any target protein for rapid screening of analog compounds. The oligomerization states of stimulator of interferon genes (STING) are explored through a multitude of experiments and several hypotheses are discussed throughout this work.
Scholar Commons Citation
Lawless, William Emmett Brian, "Methods for Analytical and Biophysical Analysis for Investigation of Stimulator of Interferon Genes in Drug Discovery" (2023). USF Tampa Graduate Theses and Dissertations.
https://digitalcommons.usf.edu/etd/10727
