Document Type
Article
Publication Date
10-2018
Digital Object Identifier (DOI)
https://doi.org/10.7554/eLife.38856
Abstract
Binary fission has been well studied in rod-shaped bacteria, but the mechanisms underlying cell division in spherical bacteria are poorly understood. Rod-shaped bacteria harbor regulatory proteins that place and remodel the division machinery during cytokinesis. In the spherical human pathogen Staphylococcus aureus, we found that the essential protein GpsB localizes to mid-cell during cell division and co-constricts with the division machinery. Depletion of GpsB arrested cell division and led to cell lysis, whereas overproduction of GpsB inhibited cell division and led to the formation of enlarged cells. We report that S. aureus GpsB, unlike other Firmicutes GpsB orthologs, directly interacts with the core divisome component FtsZ. GpsB bundles and organizes FtsZ filaments and also stimulates the GTPase activity of FtsZ. We propose that GpsB orchestrates the initial stabilization of the Z-ring at the onset of cell division and participates in the subsequent remodeling of the divisome during cytokinesis.
Was this content written or created while at USF?
Yes
Citation / Publisher Attribution
eLife, v. 7, art. e38856
This is an open-access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.
Scholar Commons Citation
Eswara, Prahathees J.; Brzozowski, Robert S.; Viola, Marissa G.; Graham, Gianni; Spanoudis, Catherine; Trebino, Catherine; Jha, Jyoti; Aubee, Joseph I.; Thompson, Karl M.; Camberg, Jodi L.; and Ramamurthi, Kumaran S., "An Essential Staphylococcus aureus Cell Division Protein directly Regulates FtsZ Dynamics" (2018). Molecular Biosciences Faculty Publications. 39.
https://digitalcommons.usf.edu/bcm_facpub/39
